Deletion of mtrC in Haemophilus ducreyi increases sensitivity
نویسندگان
چکیده
Sherri D. Rinker, 1 Michael P. Trombley, 1 Xiaoping Gu, 1 Kate R. Fortney, 2 and Margaret E. 7 Bauer 1 * 8 9 Departments of Microbiology and Immunology 1 , Medicine 2 , Indiana University School of 10 Medicine, 635 Barnhill Drive, Room MS 420, Indianapolis, IN, 46202-5124 11 12 13 14 Running title: MTR transporter-mediated AP resistance in H. ducreyi 15 16 17 18 * Corresponding author. Mailing address: 635 Barnhill Dr, Room MS 420, Indianapolis, IN, 19 46202-5124. Phone: 317-274-8143. Fax: 317-274-4090. 20 Copyright © 2011, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved. Infect. Immun. doi:10.1128/IAI.01316-10 IAI Accepts, published online ahead of print on 28 March 2011
منابع مشابه
Deletion of mtrC in Haemophilus ducreyi increases sensitivity to human antimicrobial peptides and activates the CpxRA regulon.
Haemophilus ducreyi resists killing by antimicrobial peptides encountered during human infection, including cathelicidin LL-37, α-defensins, and β-defensins. In this study, we examined the role of the proton motive force-dependent multiple transferable resistance (MTR) transporter in antimicrobial peptide resistance in H. ducreyi. We found a proton motive force-dependent effect on H. ducreyi's ...
متن کاملGenetic analysis of a pyocin-resistant lipooligosaccharide (LOS) mutant of Haemophilus ducreyi: restoration of full-length LOS restores pyocin sensitivity.
DNA sequence and Southern blot analyses were used to determine the genetic defect of a Haemophilus ducreyi pyocin-resistant lipooligosaccharide (LOS) mutant, HD35000R. The region of the HD35000R chromosome containing the suspected mutation was amplified, and sequence analysis detected a 3,189-bp deletion. This deletion resulted in the loss of the entire waaQ gene, another open reading frame tha...
متن کاملDevelopment of a rapid immunodiagnostic test for Haemophilus ducreyi.
Haemophilus ducreyi is the etiologic agent of chancroid, a sexually transmitted disease that increases the rate of transmission of human immunodeficiency virus. Chancroid ulcerations are difficult to distinguish from those produced by syphilis and herpes. Diagnosis based solely on clinical grounds is inaccurate, and culture is insensitive. Highly sensitive PCR has largely superseded culture as ...
متن کاملCarbon storage regulator A contributes to the virulence of Haemophilus ducreyi in humans by multiple mechanisms.
The carbon storage regulator A (CsrA) controls a wide variety of bacterial processes, including metabolism, adherence, stress responses, and virulence. Haemophilus ducreyi, the causative agent of chancroid, harbors a homolog of csrA. Here, we generated an unmarked, in-frame deletion mutant of csrA to assess its contribution to H. ducreyi pathogenesis. In human inoculation experiments, the csrA ...
متن کاملDifferential expression of porins OmpP2A and OmpP2B of Haemophilus ducreyi.
Haemophilus ducreyi is a strict human pathogen and the causative agent of the sexually transmitted disease chancroid. The genome of the human-passaged strain of H. ducreyi (35000HP) contains two homologous genes whose protein products have estimated molecular masses of 46 and 43 kDa. A comparative analysis of the deduced amino acid sequences revealed that these proteins share 27 to 33% identity...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2011